C-terminal hemagglutinin tag

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August undefined, 2024

WebAn HA-tag is derived from the human influenza hemagglutinin (HA) molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope … WebThe HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is …

Myc-tag and HA-tag vectors - Takara Bio

WebThe mature HA1/HA2 complex contains a membrane-proximal stalk domain containing the N- and C-terminal portions of HA1 and the entirety of HA2 and a membrane-distal receptor-binding domain containing receptor-binding and vestigial esterase subdomains (Figure 3B). WebFLAG-tag can be fused to the C-terminus or the N-terminus of a protein, or inserted within a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when FLAG-tag is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive. open space studios oswestry

HA Tag Protein Purification Sino Biological

WebThe HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA. Product Details Technical data sheet Product Details WebThese mammalian expression vectors allow you to express a protein of interest fused to either the c-Myc or hemagglutinin (HA) epitope tag. c-Myc and HA are well-characterized and highly immunoreactive tags, and thus are ideal for co-IP studies but are also easily detected via Western blot. WebAug 5, 2012 · Western blotting using an antibody against the C-terminal hemagglutinin (HA) tag of the reporter (anti-HA) or against GFP (anti-GFP) detected the 60-kDa GFP-mCherry-HA fusion protein in the... open space strategies scotland

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C Terminal Hemagglutinin Ha Tag Abcam Bioz

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human influenza virus. The HA-tag is derived from the HA-molecule corresponding to amino acids 98-106. HA-tag has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins … See more The DNA sequences for the HA-tag include: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The resulting amino acid sequence: YPYDVPDYA (Tyr … See more • Biology portal • Protein tag • SpyTag See more • Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson IA, Lerner RA, Wigler M (May 1988). "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method". Molecular and Cellular Biology. 8 … See more WebMany recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This antibody can detect either N-term or C-term HA tag. 产品编号. 产品名称. 包装. AG8057. HA Tag Rabbit Monoclonal Antibody. 50µl. AZ050. open space shop napoliWebSep 13, 2024 · the C-terminal epitope of GPR126 no change in cAMP levels was observed (Supp. Fig. S1A). ... In our receptor constructs, we inserted an N-terminal hemagglutinin tag (HA-TAG, red pentagon) immediately distal to the signal peptide and a C-terminal Flag tag (FLAG-TAG, yellow pentagon) right before the Stop codon. open space strategy city of melbourne

"WebDec 1, 2000 · Either a tag consisting of 6 histidines (6-HIS) or a tag containing the epitope Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (FLAG) was added to the C-terminus of soluble … " - C-terminal hemagglutinin tag

C-terminal hemagglutinin tag

WebNational Center for Biotechnology Information WebThe HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. ... The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. Application References (PubMed link indicates BioLegend ...

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WebThe MBP tag can be fused at the N- or C-terminus of the protein and can be easily cleaved using 3C protease. Fluorescent protein reporters Large polypeptide sequences from this … WebThe affinity tags are unique proteins/peptides that are attached at the N- or C-terminus of the recombinant proteins. These tags help in protein purification. Additionally, some …

WebRecombinant Influenza A Virus Hemagglutinin H3 protein (His tag) is a HEK 293 Protein fragment 17 to 529 aa range, ... His tag C-Terminus. Additional sequence information. Influenza A Virus Hemagglutinin H3 trimeric (A/Beijing/32/92) (H3N2) protein (Genebank #: … WebThere are three main categories of fusion tags that are used for different applications: epitope tags, affinity tags, and fluorescent tags. Epitope tags tend to be short peptide …

WebThe antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This … WebJun 25, 2013 · This suggests a post-translational processing of PfAtg7. We are able to attenuate levels of endogenous PfAtg7 through integration of a C-terminal regulatable fluorescent affinity (RFA) tag that allows for rapid destabilizion of …

WebAug 5, 2024 · These studies used strains in which SGS1 was C-terminally tagged with three or six copies of a human influenza hemagglutinin-derived epitope tag (3HA and 6HA). …

WebApr 14, 2024 · We investigated how CO4 affects the OsCERK1–OsCEBiP interaction and found that CO4 treatment reduced the coimmunoprecipitation of OsCERK1-HA (OsCERK1 fused to a C-terminal Hemagglutinin tag) with OsCEBiP-FLAG (OsCEBiP fused to a C-terminal DYKDDDDK peptide tag) in Arabidopsis protoplasts ( Fig. 3 A ). open space shopWebThe HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA. Product Details Technical data sheet Product Details open space strategy melbourneWebC Terminal Hemagglutinin Ha Epitope Tag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - … open space tewksbury maWebATCC hemagglutinin ha epitope tag Hemagglutinin Ha Epitope Tag, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more open space ringWebAug 19, 2024 · The H1N1 influenza pandemic vaccine has been developed from the A/California/07/09 (Cal) virus and the well-known high-yield A/Puerto Rico/8/34 (PR8) virus by classical reassortment and reverse genetics (RG) in eggs. Previous studies have suggested that Cal-derived chimeric hemagglutinin (HA) and neuraminidase (NA) … open space technology facilitationWebC-terminal hemagglutinin tag) (21). Cultures were preincubated with STI571 or the -secretase inhibitor L-685,458 for 2 h, then pulse labeled for 30 min with [35S]methionine [35S]cysteine Easy Tag Protein Labeling Mix (Perkin–Elmer) and chased for 2 h with inhibitors present. Media were immunoprecipitated with open space technology ostWebThe RPL22HA C-Terminal Hemagglutinin Tag Can Be Used To Immuno-precipitate Polysomes. Fig. 2 is a cartoon that outlines the Rib-oTag methodology. Crossing the … open space theatre company