Determination of aconitase activity

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August undefined, 2024

WebSep 17, 2024 · These intermediates are capable of oxidizing the cluster, which leads to iron release and consequent loss of the catalytic activity of aconitase. As the reaction of the Fe-S cluster with O 2 •-is fast (∼10 7 M-1 s-1), quite specific, and reversible in vivo, quantification of active aconitase has been used to evaluate O 2 •-formation in cells. WebOct 1, 1974 · TAA is also known to play a role in the TCA cycle where it inhibits the aconitase (ACO), the enzyme converting citrate to isocitrate via cis-aconitic acid (CAA) intermediate [34] [35] [36 ...

Reduced Expression of Aconitase Results in an Enhanced Rate of ...

WebEscherichia coli possesses two aconitases, a stationary-phase enzyme (AcnA), which is induced by iron and oxidative stress, and a major but less stable enzyme (AcnB), synthesized during exponential growth. In addition to the catalytic activities of the holo-proteins, the apo-proteins function as post-transcriptional regulators by site-specific … WebThe aconitase activity of a sample may be determined by the following equation: Aconitase Activity = B Sample Dilution Factor (milliunit/mL) T V B = Amount (nmole) of … flu deaths in canada by year

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WebThe aconitase activity was significantly depressed in the 2% O2 group (*P ⬍ 0.03, n ⫽ 6/group). ... Determination of the mechanism of free radical generation in damage in the presence of cyanosis: a new concept of controlled cardiac human aortic endothelial cells exposed to anoxia and reoxygenation. ... WebOct 14, 1997 · The aconitase activity was determined in the supernatant using two different methods. Method 1 is a coupled assay in which the formation of NADPH was … WebThe osmotic and activity coefficients of aqueous mixtures of NaCl and MgCl/sub 2/ have been determined at 25/sup 0/C by using the isopiestic method. These measurements extend from low concentrations to the crystallization limits of the mixtures. They are critically compared to published isopiestic, direct vapor pressure, and emf data for this system. flu deaths in massachusetts 2018

Identification of AcnR, a TetR-type Repressor of the Aconitase …

Biochemical elucidation of citrate accumulation in

WebJan 1, 2024 · Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease January 2024 Methods in molecular biology (Clifton, N.J.)1731:49-56 … WebAug 24, 2024 · Determination of reactivation conditions for Synechocystis sp. PCC 6803 ... The mitochondrial aconitase activity from rat AF5 cells decreased to 48% and 19% of the control in the presence of 2 ... flu deaths in manitobaWebNov 11, 2003 · Determination of Aconitase Protein Levels and Activity in the Tomato Genotypes Having demonstrated that leaves of the Aco-1 plants exhibited lower expression of the aconitase gene, we next turned our attention to determining the effect this had on the protein amount and activity. greene county anti drug coalition

"WebThe Aconitase Activity Assay kit provides a simple and direct procedure for measuring Aconitase activity in a variety of samples. Aconitase activity is determined in a coupled enzyme reaction in which citrate is converted to isocitrate by aconitase. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. " - Determination of aconitase activity

Determination of aconitase activity

FACTORS AFFECTING THE ACTIVITY OF ACONITASE - ScienceDirect

WebBeef heart aconitase has been studied by low temper- ature magnetic circular dichroism (MCD) spectroscopy in the wavelength region 300 to I900 nm. Together with parallel electron paramagnetic resonance and ac- tivity measurements, these data enable correlations between Fe-S cluster-type and enzymic activity in aconitase. WebSep 11, 2014 · In the aconitase activity assay protocol, aconitase catalyzes an equilibrium between aconitate, cis-aconitate and iso-citrate. These reactions are monitored by …

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WebDetermination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease Pedro M. Quirós Abstract Mitochondrial aconitase is a reversible enzyme that catalyzes the … WebSomething that is curious about aconitase is the fact that it uses iron as the inorganic substituent. Mutanogenic studies on aconitase have found substituting the iron ions with …

WebJan 10, 2024 · This protocol describes the determination of aconitase activity, which can provide an indirect measurement of Lon activity in vivo. The protocol is optimized for … WebDetermination of aconitase activity Cell pellets of the bioreactor samples were washed once with 10 ml washing buffer (20 mM Tris/HCl, pH 8.0) and then resuspended in 4 ml ice-cold lysis buffer (20 mM Tris/HCl, pH 8.0, 5 mM dithio-threitol). Mycelium was broken down by ultrasonic treatment in an

Web0.5M sorbitol at the concentration of 200mg/ml. Aconitase activity was determined by increase in absorbance at 340nm in the presence of 5mM citrate, 0.25mM NADP, 4mM MgCl 2, 10unit/ml of NADP-isocitrate dehydroge-nase (Murakami et al., 2006). 2.3. Determination of hydrogen peroxide and superoxide anion Determination of rosmarinic …

WebThus, aconitase activity is used as an indicator of levels of intracellular H 2 O 2 and its oxidative damage to cellular components. For example, substantially decreased …

WebApr 10, 2024 · This piece explains an activity that allows for the simple and accurate determination of the heat of vaporization, ΔH vap, of water at 100°C, and ultimately the approximate strength of a hydrogen bond in boiling water, in kJ·mol –1. The vaporization of water is an endothermic process represented by Equation 1: greene county animal sheltersWebFor quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator. Key Features. Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. greene county animal shelter tennesseeWebAconitase 2 ( ACO2) encodes the mitochondrial aconitase (ACO2), an enzyme catalyzing interconversion of citrate into isocitrate in the Krebs cycle. ACO2 mutations have been … greene county appraisal district missouriWebAconitase activity determined after 15 hours incubation at 5', pH 7.4. effect. Phosphate thus competed with the protein for the iron in the system, as well as prevented the added … greene county animal shelter xenia ohWebDetermination of Aconitase Activity—For the determination of the aconitase activity, cells from the CGXII main culture (∼25 ml) were harvested with ∼25 g of crushed ice (precooled to -20 °C) by centrifugation at 4,000 × g for 5 min. The cell pellet was resuspended in 900 μl of Tris-HCl (90 m m g; 4 °C), the supernatant was used ... greene county apartmentsWebAconitase 2 ( ACO2 ) encodes the mitochondrial aconitase (ACO2), an enzyme catalyzing interconversion of citrate into isocitrate in the Krebs cycle. ACO2 mutations have been initially associated with infantile cerebellar-retinal degeneration combining optic atrophy, retinal degeneration, severe encephalopathy, epilepsy, and cerebellar ataxia1–3; … greene county apsWebAlper Sarikaya, Aconitase 4 trans mechanism for the reversible dehydration of citric acid as well. From this determination, Gawron et al. surmised that the alpha-hydrogen of these substrates does not participate in the role of aconitase mechanism as the trans mechanism is nowhere near the proximity of the changing subunit. Additionally, the authors found … greene county animal shelter va