WebApr 29, 2024 · To further ensure that the Raman spectrum of each macromolecule discovered above is reproducible and accurate, we obtained in situ lipid and protein Raman spectra from [D 7]-glucose-labelled cells ... WebMay 11, 2024 · Coherent Raman scattering microscopic imaging of LDs. (A) Lipid droplets (LDs) store cholesterol ester (CE) and triacylglycerol (TAG) in the core, which is enclosed by a single layer of phospholipid membrane into which various proteins are anchored. ... 20 µm. Right, the ratio between deuterium-labeled lipids and unlabeled total lipids (H-C-H ...
Deuterium Raman imaging for lipid analysis - ScienceDirect
WebBy Raman imaging of DC CD vibrations at 1630 cm −1 we were able to show that D-GLA is mainly localized in lipid droplets in tumor cells, whereas in normal cells at a similar time … WebFeb 16, 2024 · We develop and apply super resolution bioorthogonal metabolic imaging platforms to visualize cellular metabolic dynamics in … chsl tournament
Deuterium-labeled Raman tracking of glucose accumulation
WebRaman-based optical imaging is a promising analytical tool for noninvasive, label-free chemical imaging of lipid bilayers and cellular membranes. Imaging using spontaneous … WebOct 2, 2012 · Figure 1. Representative Raman spectra of pure (A) d31-palmitic acid, (B) d33-oleic acid, and (C) d6-2,2,3,4,4,6-cholesterol. Most pronounced scattering intensities originate from CD2 and CD3 stretching vibrations between 2000 and 2300 cm−1. Specific CC bands are observed at 1622 cm−1 (for the deuterated fatty acids) and at 1653 cm−1 … WebHere we report a microscopy technique for the optical imaging, via the spectral tracing of deuterium (STRIDE), of diverse macromolecules derived from glucose. Based on stimulated Raman-scattering imaging, STRIDE visualizes the metabolic dynamics of newly synthesized macromolecules, such as DNA, protein, lipids and glycogen, via the … description of financial aid