WebApr 20, 2012 · Whole cell dynamic mass redistribution (DMR) assays enabled by label-free optical biosensor were then used to characterize their agonist activity in native HT-29. WebDec 3, 2024 · NPS modulates several biological functions including anxiety, wakefulness, pain, and drug abuse. The aim of this study was the investigation of the pharmacological profile of NPSR using the dynamic mass redistribution (DMR) assay. DMR is a label-free assay that offers a holistic view of cellular responses after receptor activation.
Identification of novel phytocannabinoids from - ScienceDirect
WebAug 10, 2012 · Dynamic Mass Redistribution Assay Decodes Differentiation of a Neural Progenitor Stem Cell - Sadashiva Pai, Florence Verrier, Haiyan Sun, Haibei Hu, Ann M. Ferrie, Azita Eshraghi, Ye Fang, 2012 Skip to main content Intended for healthcare professionals 0Cart MENU Search Browse Resources Authors Librarians Editors Societies Advanced … WebStem cells hold great potential in drug discovery and development. However, challenges remain to quantitatively measure the functions of stem cells and their differentiated products. Here, we applied fluorescent imaging, quantitative real-time PCR, and label-free dynamic mass redistribution (DMR) assays to characterize the differentiation process of … hideaway farms dispensary
A holistic view of GPCR signaling Nature Biotechnology
WebApr 11, 2024 · Dynamic mass redistribution assay. All DMR assays were performed using an Epic® BT system (Corning, NY, USA). Cells were directly seeded in Epic 384-well biosensor microplates. The seeding density of HT-29 was 32,000 cells/well. CHO–K1-hGPR35a and CHO–K1-mGPR35 were seeded at a density of 15,000 cells/well. After being cultured for … WebDynamic Mass Redistribution Assays F11 cells were seeded in 384-well EnSpire-LFC plates (6057408; PerkinElmer) pretreated with laminin and poly- d -lysine. Cells were seeded at a density of 10 4 cells/well in 50 µL of culture medium and grown in a humidified atmosphere containing 5% carbon dioxide at 37 °C for 24 h. WebMar 1, 2024 · Dynamic mass redistribution (DMR) assay. All DMR assays were carried out using an Epic® BT system (Corning, NY, USA). Cells were directly seeded on an Epic 384-well biosensor microplate at the optimized density (1.8 × 10 4 cells/well). After ~ 22 h incubation, cells formed a stable and confluent monolayer membrane at the bottom of the ... howell winery menu