Platinumtm sybrtm green qpcr supermix-udg
WebbPlatinum ® SYBR Green qPCR SuperMix-UDG 2 × 1.25 ml 12.5 ml 50 mM Magnesium Chloride (MgCl 2) 1 ml 2 × 1 ml ROX Reference Dye 100 µl 500 µl 20X Bovine Serum …
Platinumtm sybrtm green qpcr supermix-udg
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WebbPlatinum™ SYBR™ Green qPCR SuperMix-UDG with ROX provides a sensitive, cost-effective system for real-time PCR detection with SYBR™ Green I dye; Platinum™ qPCR … WebbPlatinum® Quantitative PCR SuperMix-UDG with ROX is a ready-to-use reaction mix for the amplification and detection of DNA in real-time quantitative PCR (qPCR) on instruments …
WebbThermo Fisher two step m mlv platinum sybr green qpcr supermix udg kit Two Step M Mlv Platinum Sybr Green Qpcr Supermix Udg Kit, supplied by Thermo Fisher, used in various … Webb23 aug. 2024 · A qPCR master mix was prepared with all common components. Volumes for a single 25 μL reaction were 12.5 μL of Platinum ® SYBR ® Green qPCR SuperMix-UDG (ThermoFisher Scientific Australia, Sydney, Australia), 1 μL each of 10 μM primer stocks specific for gene of interest ( Table 1 ), 2.5 μL of cDNA and DEPC-treated water to 25 μL.
Webb• Platinum™ SYBR™ Green qPCR SuperMix-UDG (12,5 ml) • 50 mmol Magnesiumchlorid (2 × 1 ml) • ROX Referenzfarbstoff (500 µl) • 20X BSA (1,3 ml) Es stehen ausreichend … Webb106 Two Easy PCR SuperMix were purchased from Transgen (Beijing, China). T7 RNA 107 polymerase (20 U/µL) with five T7 RNA polymerase buffers, RevertAid™ Master Mix 108 and PlatinumTM SYBRTM Green qPCR SuperMix-UDG w/ROX were obtained from 109 Thermo Fisher Scientific. (Waltham, USA). Nt.BstNBI, Bst DNA polymerase with 10
Webb28 jan. 2014 · The cDNAs were amplified by RT-qPCR in a final volume of 20 μL containing 1 μL cDNA, 10 μL Platinum ® SYBR Green qPCR Supermix-UDG (Invitrogen), and 2–5 pmol of each primer. Amplification was standardized in a 7500 Fast Real-Time Thermal Cycler (Applied Biosystems, São Paulo, Brazil) using the following conditions: 50 °C for 20 s, 95 …
WebbPlatinum® SYBR® Green qPCR SuperMix - UDG is functionally tested using plasmid DNA quantified by digital PCR to ensure robust amplification of DNA targets. Specification: The slope must be -3.4 ± 0.2. Result: Meets specification. Specification: The amplification efficiency must be > 90%. Result: Meets specification. slurry pipe flowWebbPlatinum® SYBR® Green qPCR SuperMix-UDG with BSA is a 2.5X concentration, ready-to-use reaction mix that is specifically formulated for real-time quantitative PCR (qPCR) on … solar magic colour led path light - 20 packWebbThermo Fisher platinum sybr green qpcr supermix udg with rox Platinum Sybr Green Qpcr Supermix Udg With Rox, supplied by Thermo Fisher, used in various techniques. Bioz … slurry pipeline projects in indiaWebb23 nov. 2016 · Then, quantitative RT-PCR was conducted on a CFX96 Touch sequence detection system (Bio-Rad, Hercules, CA, USA) using SYBR Green (Platinum SYBR Green qPCR SuperMix-UDG reagents; Invitrogen, Grand Island, NY, USA). GAPDH was used as the normalization control and the relative expression levels were calculated using the 2 −ΔΔ … solar mandate californiaWebbPlatinum™ SYBR™ Green qPCR SuperMix-UDG provides a sensitive and convenient system for real-time PCR detection with SYBR™ Green I dye. The SuperMix format combines … solar magnetic fieldWebb带 ROX 的 Platinum™ SYBR™ Green qPCR SuperMix-UDG 包括含 SYBR™ Green I 和 ROX 参比染料的 2X SuperMix，以及 50 mM MgCl 2 。带 ROX 的 Platinum™ qPCR … slurry pit safetyWebbqPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or... Shop Platinum™, … slurry pipeline in india