WebGram Iodine Solution: Dissolve 1 g of iodine, 2 g of potassium iodide, and 3 g of sodium bicarbonate in 300 ml of water. ... Further delay will cause excess decolorization in the gram-positive cells, and the purpose of staining will be defeated. Counterstain with 5 drops of the safranin solution for 20 seconds. WebFeb 9, 2014 · To carry out a Gram stain, the bacteria are first washed in a purple stain called crystal violet followed by iodine. The iodine and crystal violet form large complexes which bind to the cell and ...
What does the iodine do in a Gram stain? – Heimduo
WebThe gram stain, originally developed in 1884 by Christian Gram, is probably the most important procedure in all of microbiology. It has to be one of the most repeated procedures done in any lab. Gram was actually using dyes on human cells, and found that bacteria preferentially bind some dyes. The Gram stain is a differential stain, as opposed ... WebThe iodine solution allows the stain to be retained better by forming an insoluble crystal violet-iodine complex. Both gram positive and negative bacteria will retain color after this step. The iodine solution allows the stain to be retained better by forming an insoluble crystal violet - iodine complex . myofibril assembly 翻译
What does the iodine do in a Gram stain? – Heimduo
WebMar 14, 2024 · The Gram stain is a differential staining procedure that shows which bacteria are Gram-positive or Gram-negative based on their stain color. Acetone alcohol is one reagent used in this process to provide the color differentiation. Gram-positive bacteria have a thick peptidoglycan layer and stain purple, while Gram-negative microorganisms have a ... Webgram negative. List the reagents of the gram stain technique in order and their general role in the staining process. 1. Crystal violet - primary stain. 2. Grams iodine - mordant. 3. Alcohol - decolorizer. 4. WebThe function of a mordant in a Gram stain is to prevent the crystal violet from leaving the Gram-positive cell. The mordant used in the Gram stain is iodine, and when added, forms a complex with the crystal violet stain within the cell walls of Gram-positive bacteria, preventing the stain from leaving. The correct answer is (d). myofibril are composed of what